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KMID : 0624620100430120789
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BMB Reports
2010 Volume.43 No. 12 p.789 ~ p.794
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Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening
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Wang Mingke
Sun Huiqin
Jiang Fan
Han Jing
Ye Feng
Wang Tao
Su Yongping
Zou Zhongmin
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Abstract
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A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-β1 and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
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KEYWORD
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C3H/10T1/2 cells, Expression pattern, Gene trapping, Nuclear translocation, Splicing variants
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